TABLE 1.

Primers used for generating TGFBI mutations

Mutant	Primer sequencea	Changes introduced
R124H	CTCAGCTGTACACGGACCACACGGAGAAGCTGAGG	G418 to A
R124L	CTCAGCTGTACACGGACCTCACGGAGAAGCTGAGG	G418 to T
R124C	CTCAGCTGTACACGGACTGCACGGAGAAGCTGAGG	C417 to T
A546T	TTGCTCCCACAAATGAAACCTTCCGAGCCCTGCCA	G1683 to A
R555W	CCTGCCACCAAGAGAATGGAGCAGACTCTTGGGAG	C1710 to T
R555Q	CCTGCCACCAAGAGAACAGAGCAGACTCTTGGGAG	G1711 to A

^a Only the sequence of the forward primer is shown. The reverse mutation primer is complementary to the forward primer. Changes introduced are shown in bold italics. Nucleotide numbering is according to entry NM_000358.