TABLE 1.

CCR5 and CD4 expression levels at the surface of cells infected by the JRRen or the Bx08Ren viruses and IC₅₀ values for MVC or TAK779 in infection inhibition assays

The IC₅₀ values for TAK779- and MVC-mediated inhibition of infection were calculated by nonlinear regression analysis of the experimental data presented in Fig. 6. Cell surface expression levels of CCR5 and CD4 (geometric mean fluorescence intensities) were measured by flow cytometry analysis using the phycoerythrin-conjugated mAbs 2D7 and SK3 (BD Biosciences), respectively. Background staining was assessed with the appropriate isotype- and fluorochrome-matched control mAb and subtracted. Analysis was performed on a FACSCalibur flow cytometer (BD Biosciences). Results represent means ± S.D. of four independent experiments.

Cells	CCR5 (GeoMean)	CD4 (GeoMean)	Glycoprotein	Inhibitor	IC50
					nm
PBMCs	1 ± 0.6	13 ± 3	JR-CSF	MVC	1 ± 0.7
				TAK779	220 ± 90
			Bx08	MVC	1 ± 0.8
				TAK779	419 ± 75
U87-R5	13 ± 5	92 ± 40	JR-CSF	MVC	0.4 ± 0.2
				TAK779	92 ± 61
			Bx08	MVC	0.6 ± 0.3
				TAK779	146 ± 78
HEK-293T-R5	486 ± 125	7 ± 4	JR-CSF	MVC	83 ± 52
				TAK779	6018 ± 3998
			Bx08	MVC	97 ± 2
				TAK779	10629 ± 3063