TABLE 1.
Sequence comparisons of the 14 core complex I subunits from different species
Subunits are grouped according to complex I domain organization (8, 32). The names of subunits are in bovine and P. denitrificans nomenclature. Multiple sequence alignment of complex I subunits from human and species listed was performed with ClustalW2 (53). Sequence identity (and similarity in brackets) was calculated compared with human sequences.
| Domain | Subunit | Bovine | P. denitrificans | T. thermophilus | E. coli |
|---|---|---|---|---|---|
| % | % | % | % | ||
| Dehydrogenase | 51 kDa/Nqo1 | 97 (99) | 64 (77) | 43 (57) | 38 (56) |
| 24 kDa/Nqo2 | 96 (98) | 36 (52) | 33 (54) | 34 (56) | |
| 75 kDa/Nqo3 | 97 (99) | 52 (64) | 23 (39) | 16 (29) | |
| 49 kDa/Nqo4 | 95 (99) | 59 (77) | 44 (64) | 27 (42) | |
| 30 kDa/Nqo5 | 89 (94) | 51 (66) | 25 (38) | 8 (15) | |
| Connecting | PSST/Nqo6 | 86 (91) | 69 (81) | 47 (67) | 36 (51) |
| TYKY/Nqo9 | 93 (95) | 72 (82) | 31 (43) | 29 (41) | |
| ND3/Nqo7 | 74 (83) | 28 (50) | 21 (41) | 22 (41) | |
| Membrane | ND1/Nqo8 | 78 (88) | 35 (54) | 35 (51) | 35 (56) |
| ND6/Nqo10 | 62 (75) | 17 (33) | 15 (31) | 17 (36) | |
| ND4L/Nqo11 | 73 (90) | 24 (49) | 23 (42) | 19 (46) | |
| ND5/Nqo12 | 69 (81) | 28 (41) | 31 (46) | 28 (45) | |
| ND4/Nqo13 | 74 (85) | 26 (47) | 24 (40) | 26 (44) | |
| ND2/Nqo14 | 63 (81) | 14 (29) | 19 (31) | 16 (32) |