TABLE 2.
Representative purification of complex I from P. denitrificans
Starting material was 350 g of P. denitrificans cells.
| Purification step | Volume | Protein | Total activitya | Specific activity |
|---|---|---|---|---|
| ml | mg | μmol min−1 | μmol min−1mg−1 | |
| Membranes | 300 | 3000 | 4842 | 1.61 |
| Solubilized membranes | 780 | 2808 | 4064 | 1.45 |
| Q-Sepharose | 207 | 472 | 3185 | 6.75 |
| Q-Sepharose | 126 | 212 | 2331 | 11.01 |
| Mono S | 63 | 9 | 328 | 34.70 |
| Superdex 200 | 7.5 | 3.7 | 301 | 81.80 |
a The activity was measured by following the oxidation of NADH while FeCy was reduced at 30 °C. dNADH was used for membranes and solubilized membranes.