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Complex I catalytic activities in the membranes and after purification
| P. denitrificans sample | Reaction | Specific activity |
|---|---|---|
| μmol min−1mg−1 | ||
| Membranes | NADH:FeCy | 3.53 ± 0.230 |
| dNADH:FeCy | 2.06 ± 0.030 | |
| dNADH:O2 | 0.82 ± 0.069 | |
| dNADH:O2 + 20 μm piericidin A | 0.02 ± 0.003 | |
| dNADH:O2 + 20 μm rotenone | 0.05 ± 0.006 | |
| Purified complex I | NADH:FeCy | 88.29 ± 1.81 |
| NADH:DQ | 14.98 ± 0.44 | |
| NADH:DQ + 20 μm piericidin A | 1.05 ± 0.07 | |
| NADH:DQ + 20 μm rotenone | 2.05 ± 0.10 | |
| IC50 piericidin A | 0.10 μm | |
| IC50 rotenone | 0.17 μm |
