TABLE 5.
Molecular masses of subunits of P. denitrificans complex I
Masses of entire subunits were measured by LC-ESI-MS, with the standard deviation shown in parentheses. No mass measurements were obtained for subunits Nqo7 and Nqo10. Calculated masses are for strains ATCC 13543 and, in brackets, Pd 1222. For supernumerary subunits, bovine homologue name is shown in parentheses.
| Subunit | Mass |
Mass difference | Modification | |
|---|---|---|---|---|
| Observed | Calculated | |||
| Da | ||||
| Nqo1 | 47100.9 (10.5) | 47191.28 (47219.33) | −90.4 (−118.43) | −Met +acetyl |
| Nqo2 | 26124.7 (2.2) | 26122.01 (26122.01) | +2.6 (+2.72) | None |
| Nqo3 | 73002.6 (30.6) | 73159.66 (73203.66) | −157.1 (−201.06) | ? |
| Nqo4 | 46631.1 (8.1) | 46674.63 (46724.64) | −43.5 (−93.51) | ? |
| Nqo5 | 23765.6 (8.3) | 23730.98 (23892.18) | +34.6 (−126.54) | ? |
| Nqo6 | 19527.6 (1.3) | 19177.04 (19504.63) | +350.5 (+22.93) | ? |
| Nqo7 | 13633.19 (13651.23) | |||
| Nqo8 | 38725.3 (16.5) | 38751.4 (38841.56) | −26 (−116.26) | ? |
| Nqo9 | 18765.0 (0.9) | 18959.70 (18900.62) | −194.6 (−135.58) | ? |
| Nqo10 | 21819.39 (21688.19) | |||
| Nqo11 | 10855.5 (0.7) | 10856.16 (10856.16) | −0.7 | None |
| Nqo12 | 77813.7 (21.7) | 77705.77 (77735.86) | +108 (+77.88) | ? |
| Nqo13 | 56572.9 (18.4) | 56417.48 (56484.55) | +155.4 (+88.36) | ? |
| Nqo14 | 52612.1 (15.7) | 52535.40 (52535.40) | +76.7 | ? |
| PdNUMM (13 kDa) | 6924.2 (1.0) | (7058.92) | −134.7 | −Met −disulfide |
| PdNUYM (AQDQ) | 12029.7 (0.7) | (12028.40) | +1.3 | None |
| PdN7BM (B17.2) | 14415.4 (0.6) | (14413.93) | +1.5 | None |
| 14443.3 (0.6) | +29.3 | +formyl | ||