FIGURE 1.

MPTP promotes Tau protein phosphorylation in human neuroblastoma M17 cells. M17 cells treated with the indicated amounts of MPTP for 48 h were analyzed by Western blot analysis using the indicated antibodies. Based on blot band intensities, relative Tau phosphorylation was determined. A, Western blots. B, relative Tau phosphorylation. The relative Tau phosphorylation at each point was determined by normalizing the band intensity of phosphorylated Tau at the indicated site at that point with the respective total Tau band intensity. Data shown represent mean ± S.E. from three independent experiments. *, p < 0.001, and **, p < 0.005 relative to vehicle-treated control cells (t test).