FIGURE 3.

MPTP up-regulates intracellular α-synuclein protein in neuroblastoma M17 cells. MPTP or vehicle-treated cells from Fig. 1 were analyzed by Western blot analysis using the indicated antibodies. Based on blot band intensities, the relative levels of α-synuclein were determined by normalizing the α-synuclein band intensity with the respective actin band intensity. A, Western blots. B, relative amounts. The relative amount of α-synuclein at each point is presented as the fold of vehicle-treated cells. Values are mean ± S.E. from three independent experiments (*, p < 0.05; **, p < 0.005; ***, p < 0.001 with respect to vehicle treated controls (t test)). C, the correlation between Ser²⁶² phosphorylation and α-synuclein level. Relative amounts of α-synuclein are from panel B, whereas relative Tau Ser²⁶² phosphorylation values are from Fig. 1B.