FIGURE 5.

α-Synuclein stimulates Tau phosphorylation by PKA in vitro. Tau was phosphorylated by PKA in the presence of α-synuclein or BSA. At the indicated time points, aliquots were removed and analyzed by Western blot analysis. Based on blot band intensities, the relative amounts of phosphorylated Tau at each site at each time point were determined. A, Western blots. B and C, relative Tau phosphorylation at the indicated sites in the presence of the indicated proteins. Values represent mean ± S.E. of three independent experiments. *, p < 0.04; **, p < 0.001 against respective Tau phosphorylated in the presence of BSA control (t test). D, the comparison of Tau phosphorylation at the indicated sites in the presence of α-synuclein. Values are from panels B and C.