FIGURE 2.

NleC inhibits NFκB function by targeting is constituents. Fold increase in NFκB-dependent luciferase activity of Hela cells co-transfected with vectors encoding pEGFP or pEGFP-NleC and NFκB pathway components TRAF2, IKKα, or IKKβ (A) and p65 (C). Transfection with pEGFP-NleC inhibits luciferase activity associated with plasmid expression of all interrogated IKK pathway components. Data shown are mean (±S.D.) of three experiments done in triplicate with level of significance (Student's t test) indicated. ***, p ≤ 0.005 as compared with empty vector control. B, representative immunoblot probing for actin, IKK (α and β), p65, and p50 demonstrates similar levels of IKK and loading control, actin, with decreased cellular levels of p65 and p50 in pEGFP-NleC-transfected cells.