Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Dec 16;286(7):5166–5174. doi: 10.1074/jbc.M110.196840

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — Submitochondrial localization of epitope-tagged rat MTHFD2L expressed in CHO cells. Mitochondria were subfractionated as described under “Experimental Procedures.” Whole mitochondria (MT), outer membrane (OM), intermembrane space (IMS), matrix (Mat), and inner membrane (IM) fractions were resolved on 10% SDS-polyacrylamide gels and subjected to immunoblotting with anti-V5 (1:1500 dilution), anti-Hsp60 (inner membrane/matrix marker; 1:1200 dilution), anti-COX I (inner membrane marker; 1:1000 dilution), or anti-Bcl-x_S/L (outer membrane marker; 1:750 dilution) antibodies. MTHFD2L migrated at an apparent molecular mass of 37 kDa, Hsp60 migrated at 60 kDa, COX I migrated at an apparent molecular mass of 35 kDa, and Bcl-x_S/L migrated at an apparent molecular mass of 32 and 35 kDa.