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Increased AGE1 dosage alleviates age2Δ gcs1-4 temperature sensitivity. Patches of cells harboring plasmid-borne GCS1 or AGE1 (A) and AGE1ΔN or AGE1 (B) were grown on selective medium at 23 °C, replica-plated to enriched medium, and incubated at 37 °C for 1 day or at 30 °C for 2 days. For each situation, two independent yeast transformants are displayed.
