FIGURE 9.

DAG stimulates in vitro ArfGAP activity of Age1ΔN. Activation of hydrolysis of GTP-loaded myristoylated-Arf1 by Age1ΔN-His₆ was assessed in assays in which the lipid content was either 100% dimyristoylphosphatidylcholine (white bar), 85% dimyristoylphosphatidylcholine, 15% phosphatidic acid (gray bar), or 85% dimyristoylphosphatidylcholine, 15% dioleoylglycerol (black bar). The low level of background radioactivity observed in the absence of Age1 protein was subtracted from all data values. S.D. were calculated from triplicate samples.