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. 2010 Nov 22;286(7):5338–5346. doi: 10.1074/jbc.M110.100719

FIGURE 6.

FIGURE 6.

Heparan sulfate binds to recombinant Ndst1. Wild type CHO cells were transfected with mNdst1 and grown in medium containing [35S]sulfate. Recombinant enzyme was purified from the conditioned medium, and samples were analyzed for [35S]glycosaminoglycans by agarose gel electrophoresis before and after degradation with chondroitinase ABC (ChaseABC) and heparitinases I and II (Hepase). The positions of standard heparan sulfate (HS), dermatan sulfate (DS), and chondroitin sulfate (CS) are shown.