TABLE 3.
Rate constants for bimolecular and geminate recombination of CO with wild-type and L86A CerHb at pH 7, 20 °C
| CerHb | k′CO | kgem,CO | Fgem,CO | k′entry,CO |
|---|---|---|---|---|
| μm−1s−1 | μs−1 | μm−1s−1 | ||
| Leu-86(wt)a | 32 ± 5 | 80 ± 40 | 0.05 ± 0.03 | 640 ± 400 |
| Leu-86(wt) + xenon | 26 | 63 ± 10 | 0.29 ± 0.5 | 90 |
| Ala-86 | 30 | ≥150, 15 | 0.13 | 190 |
| Ala-86 + xenon | 27 | ≥150, 28 | 0.34 | 80 |
a The numbers for wt CerHb are based on the average of all determinations for the past 6 years (≥10 separate preparations, see Refs. 5, 8, and 9). The error in k′entry is very large because Fgem is close to 0.0, poorly defined, and has an error of ±60%. When Fgem is ≥0.1, the error in k′entry diminishes greatly to ∼±20%.