FIGURE 4.
BMMC apoptosis induced by secretory granule damage is cysteine cathepsin- and caspase-dependent. WT and SG−/− BMMCs (0.5 × 106 cells/ml) were either left untreated or treated for 30 min with 40 μm Z-DEVD-FMK (A and B, FMK) or for 120 min with 20 μm E64d (C and D), followed by incubation with 0, 100, or 500 μm LLME for 4 h, staining with annexin V (AnnV)/PI, and flow cytometry analysis. Data are from a representative experiment, expressed as mean ± S.E. (n = 3). E, Western blot analysis showing equal levels of cathepsin C in WT and SG−/− BMMCs.