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SG affects the secretory granule damage but not apoptosis in BMMCs deprived of growth factors. WT and SG−/− BMMCs were cultured in culture medium deprived of growth factors necessary for cell survival. At the time points indicated, cells were assessed for extent of apoptosis using annexin V (AnnV)/PI staining (A) and lysosomal damage as judged by acridine orange staining (B).
