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LLME-mediated apoptosis in BMMCs involves mMCP-4 (chymase), mMCP-6 (tryptase), and MC-CPA. BMMCs (0.5 × 106 cells/ml) from WT, mMCP-4−/−, and mMCP-6−/− mice (A); WT, MC-CPA−/−, and MC-CPAY356L, E378A mice (B) were either left untreated or incubated for 4 h with different concentrations of LLME, followed by staining with annexin V (AnnV)-FITC/PI and flow cytometry analysis. Data are from a representative experiment and are expressed as mean ± S.E. (n = 3).
