FIGURE 1.

Purification of EcfAA′T complexes. Coomassie Blue-stained SDS-polyacrylamide gels showing the purified fractions after nickel-Sepharose and size-exclusion chromatography. a, EcfAA′T was expressed in L. lactis (lane 1) or in E. coli (lane 2). b, EcfAA′T was co-produced with seven S-components in E. coli. BioY2 is not shown but behaved in the same way as BioY. The identities of the S-components were confirmed by Western blotting and detection using anti-STREPII tag antibodies (bottom panel). The Western blot was used only for qualitative purposes, and the amounts of protein loaded on the corresponding SDS-polyacrylamide gel were not the same as on the Coomassie Blue-stained gel.