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Mutations in the intra-protomer groove of MtrE impair the MtrCDE pump. Growth curves for a ΔtolC TG1(DE3) strain expressing the mtrCDE genes, in the presence of (A) 16 μg/ml nafcillin and (B) 3 μg/ml tetracycline, for wild-type mtrE and a series of mtrE mutants, and the same strain transformed with the empty vector as control. The growth curve corresponding to each mutant, the WT and control are indicated to the right of each curve.
