FIGURE 1.

Domain structure and phylogenetic analysis of the AUP1 protein family. A, AUP1 has 410 amino acids and is highly conserved among multicellular organisms. It contains four conserved domains. In the very N terminus, AUP1 has a long hydrophobic region (green). Close to that, AUP1 contains an acyltransferase domain with three conserved motifs (violet boxes) of bacterial acyltransferases. In addition, AUP1 harbors a CUE domain (red) and a G2BR domain (orange). B, phylogenetic tree and domain organization of the three related proteins hAUP1, hAMFR/gp78, yCue1p as follows: light green, transmembrane (TMD); violet, putative acyltransferase; light blue, E3 ligase; red, ubiquitin binding (CUE); orange, E2 ligase binding (G2BR).