FIGURE 5.

Trypsin sensitivity of ADAM2 and ADAM3 during spermatogenesis in +/+, Clgn^−/−, and Calr3^−/− mice. A, testis lysates (5 μg) were incubated with tosylphenylalanyl chloromethyl ketone-treated trypsin and then subjected to Western blot analysis with the indicated antibodies. Asterisks indicate the IgG that reacted with the secondary antibody. B, live germ cells collected from testes were trypsinized and then analyzed by Western blotting. The lysate prepared from 5 × 10⁵ cells was loaded in each lane. ADAM2 was not digested in the Clgn^−/− germ cells, whereas ADAM3 was not digested in the Calr3^−/− germ cells.