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. 2010 Dec 3;286(7):5639–5646. doi: 10.1074/jbc.M110.140152

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Disappearance of ADAM3 from mature Calr3^−/− sperm. A, Western blot analysis of sperm lysates. ADAM3 was not detected in Calr3^−/− sperm, whereas other sperm fertilizing proteins remained. ACE, angiotensin-converting enzyme. B, immunofluorescence staining of sperm with antibodies against ADAM1B, ADAM2, and ADAM3. Fresh sperm collected from the epididymis of mutant mice were fixed in 4% paraformaldehyde and then immunostained with the following antibodies: KS107–57 (rat anti-mouse ADAM1B), KS107–190 (rat anti-mouse ADAM2), and rabbit anti-mouse ADAM3 (25). All ADAM proteins were detected in the head region of control +/+ sperm. Whereas Clgn^−/− sperm lack all ADAM proteins, Calr3^−/− sperm lack only ADAM3.