TABLE 1.
Molecular confirmation of the 46C ES cells and its differentiated NP cells
Expression of these molecules was detected by immunofluorescent staining except for VEGFR2, which was examined by RT-PCR.
| Marker | ES cell culture | NP cell culturea |
|---|---|---|
| Oct3/4 | + | − |
| Nestin | − | + |
| CD133 | Low | + |
| SSEA1 | + | + |
| PSA-NCAM | NAb | + |
| DCX | NAb | low |
| TujI | NAb | +/− |
| NeuN | NAb | − |
| GalC | NAb | +/− c |
| GFAP | − | − |
| CXCR4 | − | + |
| c-Kit | − | − |
| VEGFR2 | NAb | + |
| c-Met | + | + |
a The d5 Sox1-GFP+ NP cells were fixed and stained for indicated protein markers.
b Markers were not tested in these cells.
c Both positive and negative cells were observed.