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DFK1012 does not block induction of IL-6, TNF-α, and IL-1β gene transcripts. A–C, RAW264.7 cells were treated with LPS (10 ng/ml) in the presence or absence of DFK1012 (DK12) for 4 h. The expression of IL-6, TNF-α, and IL-1β was examined by quantitative real-time PCR. Data were normalized to the expression of the β-actin gene. Data represent the mean ± S.D. of triplicates.
