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Production of actin filament barbed ends by 3 μm fission yeast and muscle actin monomers with Arp2/3 complex without a nucleation-promoting factor
Concentrations of ends were calculated from the time course of polymerization when half of the actin monomers were polymerized.
| Reaction | Concentration of barbed ends at t½ |
|
|---|---|---|
| S. pombe actin | Muscle actin | |
| nm | ||
| Actin only | 0.15 ± 0.01 | 0.15 ± 0.02 |
| 100 nm Arp2/3 | 0.39 ± 0.01 | 0.27 ± 0.05 |
| 500 nm Arp2/3 | 1 ± 0.12 | 0.6 ± 0.04 |
