FIGURE 3.

Involvement of iNOS in NOD2-mediated activation of Notch1-PI3K signaling. A, PP2 abolishes MDP-triggered expression of iNOS in mouse macrophages. B, mouse macrophages derived from WT or iNOS^−/− mice were treated with MDP for the indicated time periods and protein levels of NICD were analyzed by immunoblotting. C, macrophages derived from WT mice were treated with MDP, whereas that from iNOS^−/− mice were either treated with MDP or with SIN-1 and expression levels of Jagged1 were analyzed by immunoblotting. D and E, reduced (D) phosphorylation of p85 and 4EBP1 and (E) expression of COX-2 upon NOD2 stimulation by MDP in macrophages derived from iNOS^−/− mice in comparison to WT mice. The data are representative of three independent experiments. Med, medium.