FIGURE 1.

Schematic illustration of the hChAT (CHAT) constructs used in this study. wt-hChAT-GFP is the 69-kDa isoform of human ChAT fused at the C terminus with a GFP tag. NLS2-(−)-hChAT-GFP has an Ala substituted for each of Arg⁴⁸¹, Arg⁴⁸², Arg⁴⁸⁴, and Lys⁴⁸⁶ in the nuclear localization NLS2 sequence. In inactive hChAT-GFP, a Lys residue has been substituted for Glu⁴⁴¹. The amino acid residue positions are numbered to correspond to the 82-kDa human ChAT sequence, which differs from the 69-kDa isoform by the presence of a 118-amino acid extension on the amino terminus. The numbers in parentheses indicate the amino acid number in 69-kDa hChAT.