FIGURE 4.
Negative cross-talk between D1Rs and H3R receptors on ERK 1/2 phosphorylation in rat striatal slices. Slices were treated for 10 min with 10 μm SKF 81297 and/or 1 μm RAMH (a) or 10 μm RAMH and/or 1 μm SKF 81297 (b). ERK 1/2 phosphorylation was determined as described under “Experimental Procedures.” The immunoreactive bands from 10 to 24 (a) or eight to 23 (b) slices obtained from four to six animals were quantified, and values represent the mean ± S.E. of the percentage of phosphorylation relative to basal levels found in untreated slices (100%). Significant differences were calculated by one-way analysis of variance with post hoc Bonferroni's multiple tests. (** and ***, p < 0.01 and p < 0.001, respectively, as compared with 10 μm SKF 81297 in (a) or 10 μm RAMH in (b)).