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FACS analysis of parental and transfected Huh7 hepatocellular carcinoma cell lines stably expressing WT-EGFR (Huh7-EGFR) or mutant EGFR (Huh7-EGFRvIII) and SMMC-7721 cells. Cells were incubated with CH12 (black line) and C225 (gray line) followed by FITC-conjugated goat anti-human IgG (H+L) antibody. The negative control (irrelevant antibody) fluorescence is plotted on each panel (white line).
