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Immune effector function of chimeric antibody CH12. A, antibody-dependent cellular cytotoxicity on Huh7, SMMC-7721, and Huh7-EGFRvIII cells at an effector:target cell ratio of 100:1 and antibody concentrations ranging from 0.01 to 10 μg/ml. B, complement-dependent cytotoxicity activity of 0.01–10 μg/ml antibody on Huh7, SMMC-7721, and Huh7-EGFRvIII cells. The mean percent cytotoxicity of triplicate determinations is presented (Bars, S.D.).
