FIGURE 4.

CH12 treatment leads to less growth and vasculogenesis compared with C225 in SMMC-7721 and Huh7-EGFRvIII xenograft tumors. A, tumor sections were stained for Ki-67. The cell proliferative index was assessed as the percentage of total cells that were Ki-67 positive from six randomly selected high power fields (×400) in xenografts from six mice of each group. B, the qualitative analysis of cell proliferation. C, tumor sections were immunostained with anti-CD34 antibody. MVD values were analyzed by measuring the number of stained microvessels as described under “Experimental Procedures” from six randomly selected fields (×200) in xenografts from six mice of each group. D, the qualitative analysis of tumor vascularization. Data are the mean ± S.E. All data were analyzed by Student's t test. p < 0.05 was considered statistically significant.