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CH12 treatment leads to an increase in apoptosis compared with C225 in SMMC-7721 and Huh7-EGFRvIII xenograft tumors. Apoptotic cells were detected using the TUNEL assay. The apoptotic index was assessed by the ratio of TUNEL-positive cells:total number of cells from six randomly selected high power fields (×400) in xenografts from six mice of each group. Data are the mean ± S.E. All data were analyzed by Student's t test. p < 0.05 was considered statistically significant.
