Dynamics of the cell layer in experiment (A and C) and simulation (B and D). (A) The time-space plot is constructed along the horizontal line in Fig. 1C. (C) Time-space plot of the measured NADH-fluorescence in nonoscillating yeast cell layers. (Inset) NADH-fluorescence in a stirred cell solution. (B and D) Simulated time-space plots corresponding to panels A and C. Shown are variables Xi in a part of the two-dimensional array. Parameters: Jmax = 8 × 10−3, KM = 3, αmain = 3 × 10−4, n = 2, κ = 0.13, ϕ = 0.1, dX = 0.05, dY = 0.002; (B) k2 = 0.15, and (D) k2 = 0.05. Substrate is applied to the system via an initial extracellular substrate concentration at two compartments each with Xex(t0) = 187,210.