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. 2011 Feb 16;100(4):1129–1138. doi: 10.1016/j.bpj.2011.01.027

Figure 1.

Figure 1

(A) Using Maxwell constraint counting, the number of internal degrees of freedom of a protein decreases linearly as the backbone is cross-linked by distance constraints (dark coloring) until the Maxwell level, M, is reached. Beyond M, all additional distance constraints are redundant (light coloring). (B) For TTm, two basins in the free energy landscape yield two-state behavior.