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. Author manuscript; available in PMC: 2011 Feb 11.
Published in final edited form as: J Neurochem. 2009 Nov 26;112(3):784–796. doi: 10.1111/j.1471-4159.2009.06509.x

Fig. 5.

Fig. 5

Simultaneous administration of cycloheximide (CHX) with ethanol (EtOH) prevents ethanol-induced increases in StAR protein, as well as plasma and brain steroid levels, but does not affect ethanol-induced ACTH. Cycloheximide (20 mg/mL, i.p.) was administered with ethanol (2 g/kg, i.p.) and tissue was collected 60 min later. (a) Adrenal StAR protein expression was measured by western blot analysis as shown in a representative blot. StAR was normalized to b-actin and presented as % control values. (b) Plasma ACTH and (c) plasma progesterone levels. *p < 0.001 compared to control and #p < 0.001 compared to ethanol (ANOVA followed by Newman–Keuls test), n = 6 in duplicate.