Table 2.
Coagulation and thrombin generation assay parameters
| Sample* | aPTT, sec | aPTT + FVa†, sec | ETP, nM min | Time to peak, min | Peak height, nM | t½, min |
|---|---|---|---|---|---|---|
| NHP | 45 ± 1 | 39 ± 1 | 4390 ± 750 | 30 ± 8 | 260 ± 120 | ND |
| HA | 91 ± 2 | 78 ± 2 | NA | NA | NA | ND |
| HA + wt-FXa | 29 ± 1 | 23 ± 1 | 4370 ± 560 | 16 ± 1 | 210 ± 40 | 1.1 ± 0.1 |
| HA + FXaI16L | 53 ± 1 | 32 ± 1 | 4830 ± 400 | 13 ± 1 | 340 ± 30 | 49 ± 5 |
| HA + FXaV17A | 56 ± 2 | 33 ± 1 | 4750 ± 410 | 13 ± 1 | 315 ± 30 | 41 ± 5 |
| HB | 109 ± 3 | 87 ± 2 | NA | NA | NA | ND |
| HB + wt-FXa | 29 ± 1 | 22 ± 1 | 5076 ± 410 | 19 ± 2 | 253 ± 30 | 1.1 ± 0.1 |
| HB + FXaI16L | 55 ± 1 | 31 ± 1 | 5689 ± 250 | 15 ± 1 | 368 ± 20 | 57 ± 7 |
| HB + FXaV17A | 60 ± 1 | 34 ± 1 | 5694 ± 380 | 13 ± 1 | 378 ± 40 | 47 ± 9 |
| HA + I | 125 ± 2 | 105 ± 3 | NA | NA | NA | ND |
| HA + I + wt-FXa | 31 ± 1 | 24 ± 1 | 1930 ± 560 | 28 ± 5 | 70 ± 20 | 0.9 ± 0.1 |
| HA + I + FXaI16L | 68 ± 1 | 35 ± 2 | 3800 ± 300 | 26 ± 6 | 154 ± 5 | 54 ± 7 |
| HA + I + FXaV17A | 72 ± 2 | 37 ± 2 | 3500 ± 280 | 23 ± 4 | 147 ± 6 | 40 ± 9 |
The data are representative of 2-3 independent measurements. The errors in the fitted constants represent ± 2 SDs.
*
In these experiments 0.1nM FXa was used in all experiments with +FXa.
†
In these experiments, 10nM FVa was employed.
NHP, normal human plasma; HA, hemophilia A plasma; HB, hemophilia B plasma; HA + I, hemophilia A plasma with inhibitory antibody; NA, not able to determine a value; ND, not determined.