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. 2001 Feb 1;29(3):759–766. doi: 10.1093/nar/29.3.759

Figure 3.

Figure 3

Gene expression analysis of the simple and cascade systems with different effectors. β-galactosidase activity from the three systems at different concentrations of salicylate (A), 3-methylbenzoate (B), 3,5-dichlorosalicylate (C) and benzoatSe (D). Closed circles, nahR/Psal→trp′::′lacZ; triangles, xylS2/Pm→trp′::′lacZ; open circles, nahR/Psal→xylS2/Pm→trp′::′lacZ. Note that the maximun concentration used for 3,5-dichlorosalicylate in the β-galactosidase assays was 0.5 mM because concentrations >2 mM resulted in growth inhibition. (E) Immunodetection of the XylS2 product in a control culture of CC118 (lane 1), and in the cascade-containing strain CC1184S2PT32 upon incubation without inducer (lane 2), or with 1 mM 3,5-dichlorosalicylate (lane 3), benzoate (lane 4) and salicylate (lane 5).