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. 2001 Feb 1;29(3):759–766. doi: 10.1093/nar/29.3.759

Figure 6.

Figure 6

Analysis of a cascade circuit with nahR4/Psal leading to the expression of wild-type xylS in P.putida. β-galactosidase accumulation after addition of no effector (–), NahR4-specific effector (S, salicylate) or NahR4/XylS common effector (B, benzoate) at 2 mM in the cascade system (left) or a xylS/Pm→trp′::′lacZ simple circuit (right). A western blot for detection of XylS production in P.putida (nahR4/Psal→xylS/Pm→trp′::′lacZ) cultures after addition of the different effectors is shown under the corresponding graph.