Fig. 6.

Inositol auxotrophy phenotype of erg6Δ. Analysis of the inositol auxotrophy (Ino⁻ phenotype). Spotting assays were performed as described in the “Materials and methods”. 5-µl aliquots of a series of tenfold dilutions were spotted onto I⁺C⁻ and I⁻C⁻ media containing 2% agar and incubated at 30°C (a) and at 37°C (b). The ino1Δ strain is provided as an example of a strong Ino⁻ mutant