Table 4.
Allelic profiles from the stability testing.
| Isolate | VNTR1 | VNTR2 | VNTR3 | VNTR4 | VNTR5 | VNTR6 | VNTR7 |
|---|---|---|---|---|---|---|---|
| GM2212 | 211nt - 7r | 217nt - 8r | 212nt - 10r | 311nt - 10r | 312nt - 5r | 301nt - 10er | 435nt - 5r |
| GM2212-P10 | 219nt - 8r | 217nt - 8r | 212nt - 10r | 311nt - 10r | 312nt - 5r | 301nt - 10er | 435nt - 5r |
| Fc10 | 211nt - 7r | 217nt - 8r | 212nt - 10r | 311nt - 10r | 312nt - 5r | 301nt - 10er | 435nt - 5r |
| Fc14 | 211nt - 7r | 217nt - 8r | 212nt - 10r | 311nt - 10r | 312nt - 5r | 301nt - 10er | 435nt - 5r |
| Fc18 | 211nt - 7r | 217nt - 8r | 212nt - 10r | 311nt - 10r | 312nt - 5r | 395nt - 9er | 435nt - 5r |
In vivo stability was shown on passage 10 (P10) of the GM2212 isolate and in vitro stability on infected cod held at 10 (Fc10), 14 (Fc14) and 18°C (Fc18). Results are displayed with number of nucleotides in repeat marker and numbers of repeats. Two shifts were observed in GM2212, VNTR-1 after 10 passages on CHAB agar at 20°C and in VNTR-6 after passage in cod held at 18°C. The original sequence is shown in bold, whereas the mutations are in italics.
Heterogeneous repeats with Single nucleotide polymorphism (snp), noted as nucleotide number in repeat sequence (nt) and at repeat of occurrence (r); e = 2 snp C-T nt1 r4 r6