Figure 2.

Hsp protein expression and distribution in scrapie tissues. Specificity of anti-Hsp90 and anti-Hsp70 antibodies in the ovine medulla oblongata, as detected by western blotting (a). Arrowheads indicate intense Hsp70 immunostaining of spheroids observed in medulla oblongata (b). Immunohistochemical determination of Hsp70 in scrapie (c) and control (d) animals; Hsp70-positive and -negative Purkinje cells are indicated with arrows and arrowheads, respectively. Immunohistochemical determination of Hsp90 in scrapie (e) and control (f) animals; Hsp90-negative Purkinje cells are indicated by arrowheads, and Hsp90-positive cells are indicated by arrows. Significant increase in the percentage of Purkinje cells staining positively for Hsp70 (g) and Hsp90 (h) in scrapie cerebella. ** p < 0.01 (Student's t-test).