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. 2011 Jan 24;42(1):13. doi: 10.1186/1297-9716-42-13

Table 1.

Genes analysed and real time RT-PCR conditions

Genes GenBank1 Species2 Primers (5'→ 3')3 bp4 PCR conditions5
Ta [nM] r2 Slope
HSP27 BT021550 Bovine F: TGGCGCGTGTCCCTGGA 80 60 300 0.990 -3.33
R: GTGATCTCCACCACGCC 300
HSP72 AY662497 Bovine F: ACCCGCAGAACACGGTGTT 119 60 900 0.995 -3.33
R: AGGCTTGTCTCCGTCGTTGA 900
HSP73 NM_174345 Bovine F: CAACCTGCTTGGCAAGTTTGA 108 60 900 0.990 -3.20
R: GAAACATTGAGGATGCCATTGG 900
HSP90 [30] Ovine F: AGTCTGGAGGATCCCCAGACA 78 60 300 0.994 -3.32
R: GGGTCATCCTCGTCAATACCA 300

1 GenBank accession numbers of the sequences used for primer design.

2 Species of origin of the sequences.

3 Primers (F: Forward and R: Reverse) used for the gene amplification.

4 Length of the amplicon in base pairs (bp).

5 Real-time RT-PCR conditions for gene expression analyses: annealing temperature (Ta), primer concentration ([nM]), correlation coefficient (r2) and slope of the standard curve.