Table 2.
Presence of Babesia spp. in red deer whole blood samples from Glenveagh and Killarney.
| Glenveagh | |||
|---|---|---|---|
| Deer N° | +/- | species ID according to product I | species ID according to product II |
| G1 | + | 98% identical with B. odocoilei* | 96% identical with B. odocoilei, B. divergens, EU1** |
| G2 | + | 100% identical to B. divergens*** | 100% identical to B. divergens*** |
| G3 | + | 98% identical with B. odocoilei* | 96% identical with B. odocoilei, B. divergens, EU1** |
| G4 | - | ||
| G5 | + | 100% identical to B. divergens*** | Babesia sp**** |
| G6 | + | 100% identical to B. divergens*** | 100% identical to B. divergens*** |
| G7 | + | Babesia sp**** | 96% identical with B. odocoilei, B. divergens, EU1** |
| G8 | - | ||
| G9 | - | ||
| G10 | + | Babesia sp**** | 96% identical with B. odocoilei, B. divergens, EU1** |
| G11 | - | ||
| G12 | - | ||
| G13 | + | 100% identical to B. divergens*** | 100% identical to B. divergens*** |
| G14 | + | 100% identical to B. divergens*** | 100% identical to B. divergens*** |
| G15 | + | Babesia sp**** | 100% identical to B. divergens*** |
| G16 | + | Babesia sp**** | - |
| G17 | - | ||
| G18 | + | 100% identical to B. divergens*** | 100% identical to B. divergens*** |
| G19 | + | 100% identical to B. divergens*** | - |
| G20 | - | ||
| G21 | - | ||
| G22 | + | Babesia sp**** | Babesia sp**** |
| G23 | + | 100% identical to B. divergens*** | 100% identical to B. divergens*** |
| G24 | - | ||
| G25 | + | 100% identical to B. divergens*** | Babesia sp**** |
| G26 | - | ||
| G27 | + | 98% identical with B. odocoilei* | Babesia sp**** |
| Killarney | |||
| Deer N° | +/- | species ID according to product I | species ID according to product II |
| KR1 | - | ||
| KR2 | - | ||
| KR3 | - | ||
| KR4 | - | ||
| KR5 | + | 100% identical to B. divergens*** | 96% identical with B. odocoilei, B. divergens, EU1** |
| KR6 | - | ||
| KR7 | - | ||
| KR8 | - | ||
| KR9 | - | ||
| KR10 | - | ||
| KR11 | - | ||
Babesia spp were identified by sequence allignment of amplification products from PCR protocols I and II (products I and II respectively).
* sequence identical between G1, G3 and G27 (reference sequence: AY046577)
** sequence identical between G1, G3, G7, G10, KR5 (reference sequences: AY046575, AY046576, AY046577)
*** reference sequence: AY046576
**** amplicons only identifiable to genus level due to poor sequencing data