Table 1.
Primers used for qRT-PCR
| Gene ID a | Forward primer (5'-3') | Reverse primer (5'-3') | Amplicon (bp) |
|---|---|---|---|
| CYP51 | |||
| FGSG_01000 | TCTACACCGTTCTCACTACTCC | GCTTCTCTTGAAGTAATCGC | 170 |
| FGSG_04092 | CCCTTACACGATCACTACAGAC | CTCTTCGTTCCTTTAGACACAG | 186 |
| FGSG_11024 | ACTTGGTCTCACCACAGATTC | CATAGATCGGACTTCGTTTC | 187 |
| ABC transporters | |||
| FGSG_00046 | CTACTATCTGGGTGAACCTGTG | GCCACTGTTTTCAGGAGTATC | 209 |
| FGSG_02786 | CTTCGGCAAGATATGAGCTTC | GCGAGTATGGAACTCGATAGG | 161 |
| FGSG_03032 | CCTCGCAAGACAACCATACAG | AGAGTGATGTCGCGAATATCG | 176 |
| FGSG_03882 | GAACTCACGTACTCTCCTCAAC | TCGACATCAACTCTCTCTTGAC | 168 |
| FGSG_05076 | GTGCTATGCTCCAGCAATACC | CGATCAGAAGAGCGAACTGAG | 198 |
| FGSG_06771 | TCGTCAGTCCAGAAGTCAAG | TTCGGTTCACAAAGTAGTCC | 180 |
| FGSG_06881 | AAACCCCGTTGAAACAGAAC | ATCAAACCATAAGCGACGTTC | 186 |
| FGSG_07325 | CCTCTATGCTTTCCTTCATC | ATACCGTGTCTTCAACTGTCTC | 207 |
| FGSG_08308 | ACAGGCTCGTCTATTCACAG | GACCCCTTGATAACCTCTAAC | 187 |
| FGSG_08309 | GTCTCTTTGTATCAGGCTTCC | CATATTCTCTCTCCCACTCATC | 199 |
| FGSG_08312 | GATCGTCCTCTTCACTATTACC | CTCTCAGCAGTAACCATAGGAC | 183 |
| FGSG_08373 | CTGTGACGATACCGAGCTGAC | CCTGTATCGAGCCAAATCAAG | 120 |
| FGSG_08830 | GACTACTTGGCTTCTCTTTCC | CACCATCTTCTTGACTACCAAC | 250 |
| FGSG_10995 | ATTTATGACCACGCAGTCTGG | CTGCGAAAAGAGGTTCGTTAC | 121 |
| FGSG_11028 | GACACACTACCGACACAACTG | GACTGAGAAGACGAGAAGAAG | 225 |
| FGSG_11988 | GATGTAATGCTACCTGGAACAC | GATGAGACCGATTGTGAGAAC | 195 |
| Transcription factors | |||
| FGSG_00069 | GAATCTTCTCCGGCACTCAG | TTCTTCACTTCCTTCCGAGATC | 139 |
| FGSG_01293 | CCCGATATAGTCGAGCCTAAC | GGTATTTGTTGCTCCGTTGAC | 101 |
| FGSG_01341 | ATTCTGGCATGGATGATGAAG | CACCAAAGTCACTGGCATATCC | 162 |
| FGSG_01669 | TTCGACTTCTCACAGCTCAGC | TGCCTGCATGTTGTACTGGTC | 120 |
| FGSG_05949 | TTCGGCAACCATCACTCCTAG | TGCTGTTCGTTCTCTCGCACT | 100 |
| FGSG_06324 | ACAGAGTGTCGAACCAGCAAC | GGTTCTCGTGGGATGCTATC | 173 |
| FGSG_06810 | ATACCCGTCTCATGAACATCG | TCGATAACAGCCTTGGCTATG | 127 |
| FGSG_09333 | CACAGCTCAACGATGCAATG | CAAGATAAGCAAGGATGCTGTG | 101 |
| FGSG_09349 | GCTTGATTGCCCCTCTGAGA | GGTTCGCATTCTCCTGGTTC | 123 |
| FGSG_10470 | AGAGATCACCACGTCCGAAC | TGGTGGGTGTAGATGTGGTTC | 114 |
| FGSG_10914 | CATCGGGCTCTAGTACGAATC | CCAGAAAGCAGCAGTATGCTC | 141 |
| FGSG_11561 | ACTCGAAAGCATTGGATCAGC | AGACGGACGAAATCATCGTC | 130 |
a The given gene ID is the entry number of the F. graminearum genome annotation FG3 provided by Broad Institute.