Table 3.
Comparison of differential gene expression as determined by microarray hybridization and qRT-PCR
| Gene ID a | Type b | Microarray | qRT-PCR | ||
|---|---|---|---|---|---|
| log2 FC c | q d | log2 FC c | p e | ||
| CYP51 | |||||
| FGSG_01000 | CYP51B | 2.96 | 0.0303 | 2.89 | < 0.0001 |
| FGSG_04092 | CYP51A | 7.35 | 0.0061 | 6.92 | < 0.0001 |
| FGSG_11024 | CYP51C | 2.06 | 0.0385 | 1.54 | 0.0001 |
| ABC transporters | |||||
| FGSG_00046 | MRP | 3.00 | 0.0392 | 1.17 | 0.0231 |
| FGSG_02786 | MDR | 2.16 | 0.0392 | 1.64 | 0.0036 |
| FGSG_03032 | MDR (half) | -1.29 | 0.0392 | -0.99 | 0.0793 |
| FGSG_03882 | PDR | -3.65 | 0.0516 | -3.27 | 0.0560 |
| FGSG_05076 | PDR (half) | 1.94 | 0.0385 | 1.17 | 0.0249 |
| FGSG_06771 | MDR | 2.02 | 0.0392 | 1.56 | 0.0017 |
| FGSG_06881 | MDR | 1.38 | 0.0401 | 2.08 | 0.0303 |
| FGSG_07325 | MRP | 1.57 | 0.0539 | 1.42 | 0.0033 |
| FGSG_08308 | MRP | 4.07 | 0.0508 | 3.41 | 0.0310 |
| FGSG_08309 | PDR | -2.95 | 0.0509 | -2.05 | 0.0686 |
| FGSG_08312 | PDR | 2.67 | 0.0385 | 1.90 | < 0.0001 |
| FGSG_08373 | n.d. | 1.55 | 0.0395 | 1.32 | 0.0036 |
| FGSG_08830 | PDR | 6.58 | 0.0523 | 3.78 | 0.0226 |
| FGSG_10995 | MRP | 2.61 | 0.0497 | 1.30 | 0.0176 |
| FGSG_11028 | MRP | 1.44 | 0.0502 | -0.85 | 0.4518 |
| FGSG_11988 | MDR | -1.91 | 0.0509 | -1.63 | 0.0264 |
| Transcription factors | |||||
| FGSG_00069 | Zn2Cys6 | -2.14 | 0.0385 | -1.81 | 0.0207 |
| FGSG_01293 | Zn2Cys6 | 2.08 | 0.0385 | 1.91 | 0.0010 |
| FGSG_01341 | C2H2 | 1.62 | 0.0357 | 1.92 | 0.0042 |
| FGSG_01669 | Zn2Cys6 | 3.86 | 0.0392 | 3.30 | 0.0002 |
| FGSG_05949 | WING | 4.16 | 0.0385 | 3.96 | < 0.0001 |
| FGSG_06324 | Zn2Cys6 | 1.64 | 0.0385 | 1.00 | < 0.0001 |
| FGSG_06810 | Zn2Cys6 | 1.70 | 0.0387 | 1.35 | 0.0002 |
| FGSG_09333 | Zn2Cys6 | -1.22 | 0.0385 | -0.57 | 0.0121 |
| FGSG_09349 | Zn2Cys6 | -2.16 | 0.0385 | -0.98 | 0.0092 |
| FGSG_10470 | C2H2 | 4.74 | 0.0385 | 4.32 | < 0.0001 |
| FGSG_10914 | Zn2Cys6 | -1.03 | 0.0392 | -1.14 | 0.0738 |
| FGSG_11561 | Zn2Cys6 | 1.48 | 0.0387 | 1.57 | 0.0162 |
a The given gene ID is the entry number of the F. graminearum genome annotation FG3. b Classification to gene subfamilies. c The relative transcriptional differences between fungicide-treatment vs. untreated control given as log2 transformed fold-change as determined by microarray hybridization and by qRT-PCR. d Statistical assessment of transcriptional differences by ODP. The q value is a measure of the false-positive discovery rate in a given set of genes. e Statistical assessment of transcriptional differences by Student's t-test.
ALDp, adrenoleukodystrophy protein; CYP51, cytochrome P450 sterol 14α-demethylase; MDR, multi drug resistance proteins; MRP, multidrug resistance-associated proteins; PDR, pleiotropic drug resistance proteins; C2H2, Cys-Cys-His-His motif zinc finger proteins; WING, winged helix-turn-helix DNA-binding protein; Zn2Cys6, Zn(II)2-Cys6 binuclear cluster domain-type transcription factors; n.d. not defined.