Table 1.
Amplicon | Forward Primer | Reverse Primer | Amplicon Length |
---|---|---|---|
A | TGGCAAACATACGCAAGGGA | GCGAAACACACCCCTGGAAA | 275 pb |
B | GAGGACAGTTAGTGCTGTAGGGTTG | CCTCCAACGAAGTTCCCAGAAT | 547 pb |
C | TCCTTTGTAACCAGGCAAGTGG | CCCATTTCCCAGATCCTTCACTCT | 374 pb |
D | CCCAGGGTCAGAGTATATCAGTGC | CGCTGAACGTATGTCTGAGTCA | 305 pb |
E | TGGTAGGCAGAGATGTGAGGAAAG | CCTGTTGTCCTAATGCTGGTCCTA | 382 pb |
F | CTGATCTTCAGGCAATATGGCAGG | TTGGCTGATGTCGTCTGGACA | 393 pb |
G | AAGGATAGGTGCTTGGCTGACA | GCCCTAACCCTGTGTGTGTATCTTG | 504 pb |
h | GATCACGTAACAACCCTGTCAGCT | CCTTCACAGCACCCGTCATACA | 263 pb |
i | GGTGCTGGGAATTTGACTAAGGC | TAAACTCAGGTGACAGGCTACGGC | 465 pb |
j | AGGAACCAGGGAATCGAGTGCT | AACTTGCGGGTGTTCTCTCCTT | 392 pb |
k | TGTCAGCATGATGGTGGCTCA | CTCGATTTGCCATGTCCTGTCT | 252 pb |
l | GGGCTGGGTCCATATTGCTTGA | ATGCTTTGGGCTTGCCTGAAG | 373 pb |
m | ACGACTTCCCTTCCTATCCACAG | GTAGAAAGTCGTTCTGGTTGCCAC | 234 pb |
n | CCCTAATCTTGCTGTGGTTTGG | TGAGAGCTGGGCAGACATCAA | 355 pb |
O | GGTGACAGTTGACAGAGAGCCTTC | GCTCCATGCTGACCTTGAAGTCTA | 272 pb |
Sets of primers and corresponding amplicon sizes defining fifteen target sequences along the 162 kbp genomic region of chromosome 14 containing members of the rat albumin gene family.