Figure 4.
Expression of constitutively active PKA in cdk8-2 cells. (A) Western analysis was used to confirm expression of the FLAG-PKAC protein in the Ax2bsR[FLAG-pkaC] and cdk8-2[FLAG-pkaC] cell lines. Samples were collected from vegetatively growing cells of each strain and resolved on a 12% SDS-PAGE gel, transferred to a nitrocellulose membrane and probed with the α-FLAG antibody. The blot was reprobed with an α-actin antibody to control for loading. (B) Developmental phenotype of the Ax2bsR[FLAG-pkaC] and cdk8-2[FLAG-pkaC] strain. Cells were spotted onto a lawn of K. aerogenes and incubated at 22°C. Photographs were taken after 5 days. Each colony is approximately 1 cm in diameter.