Table 1. Primer sequences for PCR of ompA and VNTR loci.
Primer name | Primer sequence (5′ – 3′) | Amplicon sizea (bp) | Nucleotide positiona | Reference |
PCTM3 | TCCTTGCAAGCTCTGCCTGTGGGGAATCCT | 779,977–780,006 | Lan et al [12] | |
NRId | CCGCAAGATTTTCTAGATTTC | 1,019 | 778,988–779,008 | Lan et al [12] |
CT1291F | GCCAAGAAAAACATGCTGGT | 195,536–195,555 | Pedersen et al [10] | |
CT1291R | AGGATATTTCCCTCAGTTATTCG | 225b | 195,760–195,738 | Pedersen et al [10] |
CT1299F | TTGTGTAAAGAGGGTCTATCTCCA | 291,758–291,781 | Pedersen et al [10] | |
CT1299R | AAGTCCACGTTGTCATTGTACG | 188 | 29,1945–291,924 | Pedersen et al [10] |
CT1335F | TCATAAAAGTTAAATGAAGAGGGACT | 737,225–737,250 | Pedersen et al [10] | |
CT1335R | TAATCTTGGCTGGGGATTCA | 153 | 737,377–737,358 | Pedersen et al [10] |
OMPSeqF | GGTGTGACGCTATCAGCATGC | 779,880–779,900 | This study | |
OMPSeqR | GACCATTTAACTCCAATGTA | 718 | 779,183–779,202 | This study |
CT1291inFd | TACAAAAGTGTTGTGATAATTC | - | 195,559–195,580 | This study |
CT1299inRd | ACGAATCCTCTAAGTACGG | - | 291,908–291,926 | This study |
CT1335inRd | GGATTCAACGATGATTAAGG | - | 737,345–737,364 | This study |
OMPSeq2Rd | TATTGGAAAGAAGCICCTAA c | - | 779,417–779,436 | This study |
according to D/UW-3/CX, accession number NC 000117.
an amplicon of 510 bp was seen in some samples.
I = inosine.
Primers used for sequencing.