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. 2011 Feb 11;6(2):e16803. doi: 10.1371/journal.pone.0016803

Figure 6. The effects of NPPB and DCPIB on OGD induced pyramidal neuron death.

Figure 6

Besides the control group (in aCSF, A), the hippocampal slices were randomly divided into three groups after 25 min OGD treatment to receive the following post-OGD treatment: 1) in bath solution (B. OGD); 2) bath solution+100 µM NPPB (C. NPPB); and 3) bath solution+10 µM DCPIB (D. DCPIB). The fluorescence density of the TO-PRO-3-I staining is proportional to the neuronal death. Between the aCSF control and the OGD group, the neuronal death increased by 5.3 fold (3.2±0.6 in aCSF vs. 16.9±1.9 in OGD, n = 13). The neuronal death was reduced to 6.0 ±0.5 (n = 20) by 100 µM NPPB, and to 9.4±1.1 (n = 20) by 10 µM DCPIB. Both NPPB and DCPIB were added in the reperfusion bath solution to inhibit post-OGD VRAC. All the fluorescence intensity values are arbitrary. **. The difference between the OGD and NPPB or DCPIB groups is statistically significant at p≤0.01t. †. The difference between the NPPB and DCPIB groups is statistically significant at p≤0.05.